Coding

Part:BBa_K1997010:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dCas9 was codon optimized for expression in E.coli


Source

This part was synthesized by company.

References